Ecotoxicological assessment of cigarette butts on morphology and photosynthetic potential of Azolla pinnata.

Cigarette butts (CBs) have become the most ubiquitous form of anthropogenic litter globally. CBs contain various hazardous chemicals that persist in the environment for longer period. These substances are susceptible to leaching into the environment through waterways. The recent study was aimed to evaluate the effects of disposed CBs on the growth and development of Azolla pinnata, an aquatic plant. It was found that after a span of 6 days, the root length, surface area, number of fronds, and photosynthetic efficacy of plant were considerably diminished on the exposure of CBs (concentrations 0 to 40). The exposure of CBs led to a decrease in the FM, FV/F0, and φP0, in contrast, the φD0 increased in response to CBs concentration. Moreover, ABS/CSm, TR0/CSm, and ET0/CSm displayed a negative correlation with CB-induced chemical stress. The performance indices were also decreased (p-value ≤ 0.05) at the highest concentration of CBs. LD50 and LD90 represent the lethal dose, obtained value for LD50 is 20.30 CBs and LD90 is 35.26 CBs through probit analysis. Our results demonstrate that the CBs cause irreversible damage of photosynthetic machinery in plants and also reflect the efficacy of chlorophyll a fluorescence analysis and JIP test for assessing the toxicity of CBs in plants.

Sunlight-induced repair of photosystem II in moss Semibarbula orientalis under submergence stress.

Lower plants such as bryophytes often encounter submergence stress, even in low precipitation conditions. Our study aimed to understand the mechanism of submergence tolerance to withstand this frequent stress in moss (Semibarbula orientalis ) during the day and at night. These findings emphasise that light plays a crucial role in photoreactivation of PSII in S. orientalis , which indicates that light not only fuels photosynthesis but also aids in repairing the photosynthetic machinery in plants. Submergence negatively affects photosynthesis parameters such as specific and phenomenological fluxes, density of functional PSII reaction centres (RC/CS), photochemical and non-photochemical quenching (Kp and Kn), quantum yields (ϕP0 , ϕE0 , ϕD0 ), primary and secondary photochemistry, performance indices (PIcs and PIabs), etc. Excessive antenna size caused photoinhibition at the PSII acceptor side, reducing the plastoquinone pool through the formation of PSII triplets and reactive oxygen species (ROS). This ROS-induced protein and PSII damage triggered the initiation of the repair cycle in presence of sunlight, eventually leading to the resumption of PSII activity. However, ROS production was regulated by antioxidants like superoxide dismutase (SOD) and catalase (CAT) activity. The rapid recovery of RS/CS observed specifically under sunlight conditions emphasises the vital role of light in enabling the assembly of essential units, such as the D1 protein of PSII, during stress in S. orientalis . Overall, light is instrumental in restoring the photosynthetic potential in S. orientalis growing under submergence stress. Additionally, it was observed that plants subjected to submergence stress during daylight hours rapidly recover their photosynthetic performance. However, submergence stress during the night requires a comparatively longer period for the restoration of photosynthesis in the moss S. orientalis .

A comprehensive review on triple R eco-management strategies to reduce, reuse and recycle of hazardous cigarette butts.

Cigarettes are the globally consumed product that contributes to public health problems and is the source of the most prevalent form of litter in the world, Cigarette butts. Cigarette butts are a major source 4000 toxic chemicals, affecting the health of wildlife, humans, and the environment and their decomposition can take years due to the resistance of cellulose acetate to bacterial and fungal degradation. In 2016, the world production of cigarettes exceeded 5.7 trillion, with the majority of them consisting of cellulose acetate filters. Consequently, a massive amount of hazardous waste leaches out in the environment. Incineration and landfilling are methods of disposal, but they can result in the emission of harmful fumes and be costly. To combat this environmental issue, researchers have explored the recycling of cigarette butts in various materials, including asphalt concrete, fired clay bricks, and as a carbon source, among others. Various approaches can be used to reduce cigarette butts pollution, but efficient collection logistics by consumers remains a crucial factor for successful recycling. This paper provides innovative solutions to mitigate the cigarette butts litter problem and the feasibility of recycling methods. Despite recent progress in cigarette butts recycling solutions, there is still much room for research in this area.

Cigarette: an unsung anthropogenic evil in the environment.

The world's population is growing steadily, and this trend is mirrored by a sharp rise in the number of people who smoke cigarettes. Instead of properly disposing of their cigarette waste, most people simply toss them aside, leading to serious environmental consequences. According to previous statistics, in 2012 alone, 6.25 trillion cigarettes were consumed by 967 million chain smokers. Past studies have shown that up to 30% of global litter is made up of cigarette waste. These discarded cigarette butts are non-biodegradable and contain over 7000 toxicants such as benzene, 1,3-butadiene, nitrosamine ketone, N-Nitrosonornicotine, nicotine, formaldehyde, acrolein, ammonia, aniline, polycyclic aromatic hydrocarbons, and various heavy metals. These toxicants have a negative impact on the habitats of wildlife and can cause serious health problems such as cancer, respiratory disorders, cardiac issues, and sexual dysfunction. Although it is still unclear how littered cigarettes affect plant growth, germination, and development, it is clear that they have the potential to harm plant health. Just like single-use plastic, trashed cigarette butts are a critical new rising form of pollution that requires scientific attention for effective recycling and disposal management. It is important to properly dispose of cigarette waste to protect the environment and wildlife, as well as to prevent harm to human health.

Effect of different waterlogging periods on biochemistry, growth, and chlorophyll a fluorescence of Arachis hypogaea L.

Peanut is among the main oil crops in India with huge economic importance. The unpredictable rainy season during the growing time of peanuts causes waterlogging in peanut fields. Waterlogging triggers major environmental limitations that negatively affect the growth, physiology, and development of peanuts. Thus, the export and production of peanuts are severely affected by waterlogging. Therefore, the understanding of metabolic mechanisms under waterlogging is important to future water-stress tolerance breeding in peanuts. This study aimed to evaluate how peanuts responded to various waterlogging conditions in terms of their development, metabolic processes, and chlorophyll fluorescence characteristics. The evaluations were carried out at different stages of peanut variety DH-86 treated with waterlogging. The peanut plants were subjected to different waterlogging periods of 20, 40, 60, 80, and 100 days. The growth parameters including total dry mass, total leaf area, and total leaves number were calculated in all treatments. The phenomenological and specific energy fluxes and maximum photosystem II efficiency (FV/Fm) were also determined. The measurements were done statistically using PCA, G-Means clustering, and correlation analysis to explore the interaction between different physiological parameters. The waterlogging for 100 days caused a significant reduction in the total number of leaves, dry mass, and total leaf area. The most sensitive parameters are specific and phenomenological energy fluxes and Fv/Fm, which notably decreased as waterlogging duration increased. The results indicated the growth and physiological performance of the peanut cv. DH-86 was affected significantly due to waterlogging and the interaction between all these parameters in waterlogging. This research focused on how peanuts respond to waterlogging stress and provides the basis for future plant breeding efforts to improve peanut waterlogging tolerance, especially in rainy regions. This will improve the sustainability of the entire peanut industry.

Effect of continuous light on antioxidant activity, lipid peroxidation, proline and chlorophyll content in Vigna radiata L.

Longer photoperiod in form of continuous light (24-h photoperiod without dark interruption) can alter the various physiological and biochemical processes of the plant. This study aimed to evaluate the effects of continuous light on various biochemical parameters associated with the growth and development of Vigna radiata L. (mung bean). The findings showed that leaf size and chlorophyll content of seedlings grown under continuous light were significantly greater than control plants subjected to 12h light/12h dark (12/12h). The activity of antioxidant enzymes superoxide dismutase (SOD, 30.81%), catalase (CAT, 16.86%), guaiacol peroxidase (GPOD, 12.27%), malondialdehyde, (MDA, 39.31) and proline (14.81%) were notably higher in 24/0h light period than 12/12h light period grown seedling at an early stage (on Day 6) while they were constant at the later stage of development. Increased activity of amylase and invertase reveals higher assimilation and consumption of photosynthetic products. This study revealed that plants were stressed at first. However, they gradually became acclimated to continuous light and efficiently used the excess light in carbon assimilation.

Tolerance and decolorization potential of duckweed (Lemna gibba) to C.I. Basic Green 4.

With growing human culture and industrialization, many pollutants are being introduced into aquatic ecosystems. In recent years, dyes have become a major water pollutant used in the manufacture of paints and other production purposes. In this research, the potential of duckweed (Lemna gibba) plant was investigated spectrophotometrically as an obvious bioagent for the biological decolorization of the organic dye C.I. Basic Green 4 (Malachite Green, BG4). Photosynthetic efficiency analysis showed that the photosynthetic apparatus of L. gibba is very tolerant to BG4. Significant induction of reactive oxygen species (ROS) scavenging enzymes was observed after 24h of biodecolorization process in L. gibba treated with 15 and 30 mg/l BG4. The experimental results showed that L. gibba has a strong ability to extract BG4 from contaminated water and the best results were obtained at 25-30°C and pH 8.0. We conclude that duckweed L. gibba can be used as a potent decolorization organism for BG4.

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions.

An efficient and reproducible protocol for in vitro propagation of Dolichandra unguis-cati has been established for the first time from nodal segments. In order to enhance survival rate under ex vitro conditions, photosynthetic potential of in vitro grown plantlets was also studied through JIP test based analysis of polyphasic OJIP chlorophyll a fluorescence OJIP transients, density of active reaction centers, light harvesting efficiency, electron transfer rate, dissipation energy, maximum quantum yield of primary PSII photochemistry and photosynthetic performance index. The best morphogenetic in term of explants response (92.2 %), shoot number (3.43 ± 0.07) and shoot length (4.7 ± 0.31 cm) was obtained on Murashige and Skoog medium supplemented with 0.5 mg l-1 BAP and 1.0 mg l-1 TDZ. The shoots exhibited high frequency rhizogenesis on half strength medium augmented with 2.0 mg l-1 IAA. In vitro plantlets developed highest rate of photosynthesis on day 18 after the initiation of rhizogenesis. High survival rate (96.16%) under ex vitro conditions was observed when in vitro plantlets having high photosynthetic efficiency (Fv/Fm > 0.75) were subjected to hardening and acclimatization process. Plantlets with reduced photosynthetic performance exhibited low survival rate under natural conditions. The developed in vitro protocol will be useful for genetic improvement and multiplication of D. unguis-cati. The results of this study also show that photosynthetic screening of in vitro developed plantlets is highly essential after the rhizogenesis process to achieve higher survival rate under field conditions.